Octalone carbodylic acids and their derivatives

ABSTRACT

1. A PHARMACEUTICAL COMPOSITION IN DOSAGE UNIT FORM COMPRISING A PHARMACEUTICAL CARRIER AND FROM 50 TO 500 MG. OF A COMPOUND HAVING THE FORMULA   3,3-DI(CH3-),6-R,7-Y,8-X-1,2,3,4,5,6,7,8-OCTAHYDRO-   NAPHTHALEN-1-ONE   WHEREIN R IS PHENYL OR PHENYL BEARING FROM ONE TO TWO SUBSTITUENTS SELECTED FROM ALKOXY HAVING UP TO FOUR CARBON ATOMS, HALOGEN AND HUDROXYL AND WHEREIN ONE OF X AND Y IS CARBOXYL AND THE OTHER IS THE GROUP   -CO-N(-R1)-R2   IN WHICH R1 AND R2 ARE HYDROGEN OR LOWER ALKYL HAVING UP TO FOUR CARBON ATOMS.

United States Patent Int. on. elm 65/20 US. Cl. 424-317 6 Claims This application is a division of our copending application, Ser. No. 389,489 filed Aug. 13, 1964.

The present invention concerns a novel class of chemical products having utility as chemical intermediates and having biological activity, especially choleretic properties. These products comprise the 6,6-dimethyl-A -8-octalone carboxylic acids corresponding to Formula I, the metal salts, and carboxyl derivatives thereof.

in which R is selected from the group consisting of pyridyl, phenyl,

and phenyl hearing from one to two substituents selected from alkoxy having up to 4 carbon atoms, halogen, and hydroxyl;

X and Y are selected from the group consisting of hydrogen, phenyl, carboxyl, lower carbalkoxyl having up to carbon atoms, carboxamide groups of the formula C ON and together form a substituent selected from the group consisting of COOCO, and

CO}|ICO RI no more than one of X and Y being selected from hydrogen and phenyl, where is selected from the group consisting of piperidino, morpholino, piperazino, and amino, wherein R and R are selected from the group consisting of hydrogen and lower alkyl having up to 4 carbon atoms, and

R is selected from the group consisting of hydrogen, lower alkyl having up to 4 carbon atoms, and di-lower alkylaminoalkyl having up to 4 carbon atoms in the alkyl portions thereof.

The products conforming to the invention, therefore, include mono-acids, di-acids, and their functional derivatives including amides, esters, and imides.

Synthetic methods useful for the preparation of these substances are illustrated in Equations 1, 2, 3, 4, and 5 which are shown below. Specific examples of biologically active products envisaged by this invention are given later, together with processes for preparing them.

In order to obtain the derivatives (I) according to the invention, it is necessary to obtain two groups of inter- 3,557,291 Patented Jan. 19, 1971 mediate products, the dienones (II-a), and the octalone anhydrides (II-b) which are obtained from the dienones. The latter serve as intermediates for the di-acid, ester, amide, and imide types of Formula I.

CH3 CH3 CH3 CH3 R-CHO our =0 RCH=CHk/= CH3 CH Intermediate dienones (IIa).-These products have the general formula shown in the table of formulas given above, in which R has the same significance as given above.

The intermediate dienones (II-a) are prepared by reacting aldehydes with 3,5,S-trimethylcyclohexenone (isophorone) in the presence of a warm dilute aqueous alkaline solution according to Equation 1.

It has been proven that in the case of benzaldehyde, the compound obtained indeed has the styryl cyclohexenone structure shown (Conia and OLeary, C.R., 249, 1002 (1959); Bull. (1960) 212).

EXAMPLE 1 A mixture of 350 g. of isophorone, 260 g. of benzaldehyde, and 2.0 l. of aqueous sodium hydroxide is refluxed with stirring for 5 hours. The mixture is allowed to cool and is neutralized with acetic acid; the crystalline product is collected and washed with water; there is thus obtained 480 g. of 3-styryl-5,5-dimethyl-2-cyclohexene-1- one, M.P. 78 C. (see Conia and Leary (loc. cit.) and Cornubert and Borel, Bull. (1929) 1158).

EXAMPLE 2 A mixture of 140.5 g. of p-chlorobenzaldehyde, 140 g. of isophorone, and 700 ml. of 5% aqueous sodium hydroxide is refluxed for 5 hours. The mixture is allowed to cool and is then neutralized with acetic acid; the product is dried and recrystallized from 300 cc. of boiling isopropanol. There is thus obtained 100 g. of 3-p-chlorostyryl-5,S-dimethylcyclohex-Z-en-l-one, M.P. 114 C.

Other intermediate dienones are given in Table 1 below, in which the compound obtained in Example 2 is listed as No. 5:

TABLE 1.DIENONES OF FORMULA II-a Solvent of Reaction of the dienones (II-a) with dienophiles such as maleic anhydride and acrylic acid leads to derivatives of A -8-octalone, resulting from displacement of the double bond from its transitory position 4[structure [II-b'] in brackets] to position 9 corresponding to structure (IIb) (double bond conjugated with the carbonyl). Proof of the position of the double bond has been obtained by infrared spectral studies, by ultraviolet studies, and by nuclear magnetic resonance.

EXAMPLE 3 A mixture of 5.12 g. of 3-(o-methoxystyryl)-5,5-dimethylcyclohex-2-en-l-one (No. 2 in Table 1) and 2 g. of maleic anhydride is heated for 2 hours on a boiling water bath. The product so obtained is dissolved in a minimum of hot benzene. On cooling, there crystallizes 5.9 g. of the anhydride 3-(o-methoxystyryl-6,6-dimethyl-A -8- octalone-1,2-dicarboxylic acid, M.P. 167-168 C.

There is thus obtained compound No. 13 of Table 2 which follows and in which are given other examples of these intermediate anhydrides.

The anhydrides are crystalline substances, soluble in most organic solvents and giving the usual reactions of anhydrides of 1,2-dicarboxylic acids. The properties of the new intermediate compounds prepared are summarized in Table 2.

TABLE 2.OCTALONE-1,2DICARBOXYLIC ANHY- DRlDES OF FORMULA IIb Solvent of crystallization Ethanol, ethyl acetate. Benzene, ethanol. Benzene.

D0. Ethanol.

15... (3,4) (CH)2C5H3...

R M.P., C. crystallization Comments Number:

1 (9011300 114 68 Cyclohexane, B.P.

isopropyl ether. (2) CH3O C H 125-126 Isopropanol (3) CH3O CGHQ 64 Isopropyl ether (3 4)(CH30)zC5H3 104 Ethanol 01cm. 113-114 Isopropanol G (2)1'IOCaH4 168 .dO 7 (3)HOCBH4 205 Ethanol, acetic ac 8 (2,5) (CHaO) C1-CsH3 101 Isopropanol 9 2-pyn'dyl 84 Dilute ethanol. Hydrochloride.

1 228-233 C./1.5 mm. 2 Recrystallized from isopropanol, M.P. 184.

No'rE.-The number preceding the radical R indicates the carbon atom to the ring subsittuted with this radical.

tants. In many cases, two isomeric acids are obtained, starting from the same reactants, according to the conditions of the reaction. These are stereoisomers which differ in the respective positions of the R and COOH groups, in relation to the ring. Both the cis and trans forms of these products are covered by the present invention. The various compounds of this group more specifically covered by the invention are given in Table 3.

TABLE 3.OCTALONE2CARBOXYLIC ACIDS OF FORMULA I-c Methanol 1 Conditions of the Reaction: a-Heating 3-4 hours on an oil bath kept at 130-140 0.; bThe oil bath is heated gradually to 250 C. and is kept at this temperature for 4 hours.

2 Ethyl ester, M.P. 97 0., and its oxirne, M.P. 179 0. Methyl ester, M.P. 101 C. The esters are obtained by esterification of the acid with a mineral acid, or by addition of the diene to the corresponding acrylic esters. The conditions of the reaction have been indicated for identification oi the derivative, difierent reaction con- (litions leading to ditierent isomers.

The compounds thus obtained are well crystallized solids, showing the general reactions of carboxylic acids, and especially can be titrated by acidirnetry. Their salts with the alkali metals are soluble in water. Other metal salts, and salts with other inorganic and organic bases are prepared by neutralization in conventional fashion.

EXAMPLE 4 3-styryl-5,5-dimethyl cyclohex-2-en-1-one, g., acrylic acid, 5.0 g., and 0.1 g. of hydroquinone are refluxed for 3-4 hours (until a sample of the reaction mixture becomes soluble in dilute aqueous sodium hydroxide). The mixture is allowed to cool and the product is crystallized by addition of water. The material is filtered, washed with water, and recrystallized from acetic acid or ethanol. There is thus obtained 6.8 g. of 3-phenyl-6,6-dimethyl-A 8-octalone-2-carboxylic acid, M.P. 212214 C.: (compound No. 16 in Table 3).

EXAMPLE 5 o-Methoxybenzylidene isophorone (compound No. 2, Table 1), g., acrylic acid, 9 g., and 0.1 g. of hydroquinone are heated for 3 hours on an oil bath kept at 130140 C. The mixture is allowed to cool and is then dissolved in 2 N aqueous sodium hydroxide solution. The alkaline solution is extracted with ether and the product precipitated as an oil with hydrochloric acid. The product so obtained solidifies on standing. It is collected, washed with water, and recrystallized from isopropanol. There is thus obtained 16.8 g. of cis 3-o-methoxyphenyl-6,6-dimethyl-A -8-octa-lone-2-carboxylic acid, M.P. 164 C. (compound No. 19, Table 3).

EXAMPLE 6 o-Methoxybenzylidene isophorone, 2 0 g., acrylic acid, 9 g., and 0.1 g. of hydroquinone are heated on an oil bath. The temperature of the oil is raised gradually to 250 C. and is kept at this temperature for 4 hours. The mixture is then treated as in Example 5 and there is obtained 12 g. of the trans isomer of that in Example 5, M.P. 208 C. (compound N0. 20, Table 3).

A special case is that of 3-(2-hydroxystyryl)-5,5-dimethylcyclohex-Z-en-l-one (compound No. 6, Table 1).

Reaction of this dienone with acrylic acid at a temperature of 130140 under reflux on an oil bath leads to the lactone of 3-(o-hydroxyphenyl)-6,6-dimethyl-A -8- octalone-Z-carboxylic acid (No. 26), M.P. 190 C. (after recrystallization from ethanol) (oxime M.P. 243 C.).

With cinnamic acid, reaction with the dienones (IIa) gives carboxylic acids of Formula I-d or 'I-d', the reaction being indicated by Equation 4 in the table of formulas.

The following compounds have thus been prepared: No. 27, R=C H M.P. 215 C. (after recrystallization from ethanol); No. 28, R=(3,4) (CH O) C H M.P. 168 C. (d).

EXAMPLE 7 Cinnamic acid, 15 g., 3-styryl-5,S-dimethyl-Z-cyclohexene-l-one, 23 g., and 0.1 g. of hydroquinone are heated for 5 hours at 180 C. The mixture is allowed to cool and is dissolved by warming in its volume of benzene; on cooling there crystallizes 5 g. of cinnamic acid, which is removed by filtration. The filtrate is extracted with 2 N NaOH and the 1,3-diphenyl-6,6-dimethyl-A -8-octalone-2- carboxylic acid or its isomer of Formula I-d is precipitated by addition of hydrochloric acid.

Di-acids.These correspond to Formula I in which X=Y=COOH. They are represented by Formula I-e. These compounds result from hydrolysis of the intermediate anhydrides (II-b) alkaline medium.

Table 4 contains data on representative examples of such derivatives:

TABLE 4.OCTALONE-l,2-DICARBOXYLIC ACIDS (I-e) EXAMPLE 8 The anhydride of 3-(o-methoxyphenyl)-6,6-dimethyl- A -8-octal0ne-1,2-dicarboxylic acid, 2.0 g., prepared as in Example 3 (No. 13), and 40 cc. of 2 N sodium hydroxide are heated until a homogeneous solution is obtained. The mixture is allowed to cool and is acidified with hydrochloric acid. The product which precipitates is collected on a filter and washed with water. It is recrystallized from aqueous ethanol. There is thus obtained 20 g. of 3-(o-methoxyphenyl) -6,6-dirnethyl-A -8-octalone 1,2 dicarboxylic acid, M.P. 235240 C. (No. 31, Table 4).

Esters.These compounds are prepared by reaction of the anhydrides (II-b) with alcohols (or with phenols). One can thus obtain either monoor diesters.

In certain cases, the condensation with dienophiles, which leads to the anhydrides (IIb), and the esterification can be carried out in a single step.

EXAMPLE 9 Fifteen grams of the anhydride of 3-phenyl-6,6-dimethyl- A -8-0ctalone-1,2-dicarboxylic acid (No. 10, Table 2) is dissolved in cc. of anhydrous ethanol; gaseous hydrogen chloride is then passed into this solution to saturation in the cold. The mixture is allowed to stand overnight and is then refluxed for 4 hours. The excess of alcohol is removed under vacuum and the residue is taken up in ether and water; it is washed with a solution of 5% sodium carbonate solution and then with water. The ethereal solution is dried over anhydrous sodium sulfate and the solvent is removed by distillation. The crystalline residue is recrystallized from petroleum ether to obtain 10 g. of 3-phenyl-6,6-dimethyl-A -8-octalone-1,2-dicarboxylic acid diethyl ester, M.P. 82 C. (No. 34).

7 EXAMPLE The anhydride of 3-phenyl-6,6-dimethyl-A -8-octalone- 1,2-dicarboxylic acid (No. 10, Table 2), 5 g., is dissolved in cc. of anhydrous acetone; there is added to this solution 1.8 cc. of fl-dimethylaminoethanol. The reaction mixture becomes hot and is then refluxed for 1 hour; the solvent is evaporated and the residue is triturated with anhydrous ether. The product is recrystallized from isopropanol by the addition of ether. There is thus obtained 4.1 g. of the Z-(B-dimethylaminoethyl)ester of 3-phenyl- 6,6-dimethyl-A -8-octalone 1,2 dicarboxylic acid or its mulas, and the important physical properties of certain representative products. In this table, the letters a, b, and 0 have the following meanings:

(a) Preparation carried out in aqueous medium With an excess of amine (more than 2 molar equivalents).

(b) (Solvent) Preparation carried out in organic medium with excess of amine (more than 2 molar equivalents).

(c) (Sol-vent) Preparation carried out in organic medium using one mole of anhydride to one mole of amine.

TABLE 5.OOTALONE 1,2-DICARBOXYLIC ACID MONOAMIDES OF FORMULAS I-g AND I-g' R1 N R R2 Technique M.P., C. Solvent of recrystallization NI'Ig b (acetone) 190-192 Acetone. NH-CH; a, b (acetone) 173-175 Ethanol. NHCH(CH )Z b (acetone) 200-205 Ethyl acetate, isopropanol. NHC H (4). b (acetone) 180-182 Ethanol.

i NH-O-CH b (acetone), b (0112012), 0 (CH Cl2) 195-200 Chlorofonn isopropanol.

42 0611 c (ethyl acetate) 180 Ethyl acetate.

43 (EH5 N (0113);. a, b (acetone) 176-180 Isopropanol.

44 CaHir N(C2H5)2 a, b (acetone), b (CHzCls), 0 (0112613) 195-200 Isopropanol ethyl acetate.

45 (EH N c (ethyl acetate) 180-182 Ethyl acetate.

46 C0115 N b (acetone) 148-150 Isopropanol.

47 CsH N N-NCH; c (ethyl acetate) 236 EthanolJ 48 (2) OH3O C5114 N(C2H5)2 b (acetone) 210 Isopropanol. 48A. (3) OH O CuH4 Same as above b (CH2C12) 180-185 Do. 49 (4) CHKOCtlH-l ..do b (acetone) 174 Ethanol.

l Soluble in both acid and alkaline medium.

isomer, the 1 (fl-dimethylaminoethyl)ester (No. EXAMPLE 12 M.P. 168-170 C.

In the case of 3-(o-hydroxystyryl)-5,5-dimetl1ylcycloheX-2-en-1-one addition of maleic anhydride and esterification of the phenolic hydroxyl group occur simultaneously.

EXAMPLE 11 (Condensation and esterification in a single step) 3-(o-hydroxystyryl) 5,5 dimethylcyclohex-2-en-l-one (No. 6, Table 1), 8.6 g., maleic anhydride, 3.2 g., and cc. of toluene are refluxed together. The reaction mixture is allowed to cool and is then filtered, and there is thus obtained 7.8 g. of the lactone of 3-(o-hydroxyphenyl)-6,6- dimethyl-A -8-octalone-1,2-dicarboxylic acid, M.P. 236 C. (No. 36).

Amides.The reaction of the anhydrides (II-b) with primary or secondary amines leads to amide acids (Ig or I-g of Equation 5). R and R have the definition given above.

Compounds (I-g) or (I-g) so obtained have the characteristics of carboxylic acids and of amides and, in particular, can be titrated by acidimetry. As the compounds F obtained are frequently mixtures of isomers, they show a rather large range of melting point. The preparation can be carried out in aqueous medium or in an organic solvent, using one or more molar proportions of the amine.

Table 5 gives the conditions for preparation, the for- The anhydride of 3-phenyl-6,6-dimethyl-A -8-octalone= 1,2-dicarboxylic acid, 10 g., is dissolved in 50 cc. of anhydrous acetone. The mixture is chilled in an ice bath, and a current of ammonia gas is passed in until an alkaline pH is evident. The product is filtered and the pasty material is washed with acetone. It is dried in air and then dissolved in Water. The mixture is filtered and acidified with hydrochloric acid solution; the precipitate is collected, washed with water, and dried in vacuo. There is thus obtained 7 g. of 3-phenyl-6,6-dimethylA -8-octalone- 2-carboxamide-l-carboxylic acid (or 1-carboXamide-2- carboxylic acid), M.P. 190-192 C. (No. 37).

EXAMPLE 13 The anhydride of 3-phenyl-6,6-dirnethyl-A -8-octalone- 1,2-dicarboxylic acid, 10 g., is dissolved in 50 cc. of acetone and there is added to this solution with cooling in an ice bath and stirring, 20 g. of a 33% aqueous solution of monomethylamine. The mixture is stirred for 1 hour. The solution becomes homogenous and then partially crystallizes; the solid is redissolved with cc. of water. The solution is acidified with hydrochloric acid; the crystalline precipitate is collected and washed with Water. It is recrystallized from ethanol to give 7.8 g. of 3-phenyl-6,6- dimethyl-A -8-octalone 2 N methylcarboxamide-l-carboxylic acid or the 1-N-methylcarboxamide-2-carboxylic acid, M.P. 176 C. (No. 43).

9 EXAMPLE 14 The anhydride of 3-phenyl-6,6-dimethyl-A -8-octalone- 1,2-dicarboxylic acid, 3.24 g., is dissolved in 7 cc. of ethyl acetate. There is added 0.85 cc. of piperidine and the exothermic reaction is allowed to proceed; the mixture is allowed to stand until crystallization occurs and the product is collected by filtration. There is obtained 2.6 g. of 3-phenyl 6,6 dimethyl-A -8-0ctalone-2-( l-piperidinocarbonyl)-1-carboxylic acid or the 2-carboxylic acid-(1- piperidinocarbonyl) compound, M.P. 170 C. (No. 45). Crystallization of this compound from ethyl acetate raises the melting point to l80-182 C.

Imidies.The action of primary amines, R -NH on the anhydrides (IIb) gives rise, after dehydration, to the imides (Ih) shown in Equation 6.

The following representative examples of these compounds are given:

EXAMPLE 15 The anhydride of 3-phenyl-6,6-dimethyl-A -8-octalone- 1,2-dicarboxylic acid, 50 g., is suspended in 80* cc. of xylene and there is added slowly and with cooling 12 g.

of n-butylamine. The reaction mixture is refluxed for 6 EXAMPLE 16 The anhydride of 3-phenyl-6,6-dimethyl-A -8-octalone- 1,2-dicarboxylic acid, 15 g., is treated with cooling with 4.5 g. of N,N-dimethylethylenediamine. The mixture is heated for 2 hours at 180 C. It is allowed to cool and is then dissolved in ether. The ethereal solution is washed with water and is extracted with dilute hydrochloric acid. The acid solution is made basic with sodium hydroxide and the precipitated oil is taken up in ether; this solution is dried over calcium chloride, and the hydrochloride is precipitated by passing in anhydrous hydrogen chloride gas. The product so obtained is recrystallized from 90% isopropanol to give 6.5 g. of the hydrochloride of 6,6- dimethyl 3 phenyl A -8-octalone-1,2-[N-(fl-dimethylaminoethyl) ]dicarboximide, which melts with decomposition about 280 C. (No. 51).

The compounds of the present invention are useful as chemical intermediates in the synthesis of biologically active end products and as pharmaceutical products in their own right, in view of their antibacterial, choleretic activity, and ability to reduce blood cholesterol concentrations.

As an example of one compound of the present invention having utility as a chemical intermediate, 6,6-dimethyl 3 phenyl A -8-octalone-1,2-dicarboxylic acid anhydride may be mentioned. This anhydride reacts with water, alcohols, amines, and other organic compounds to provide a variety of the corresponding carboxylic acid derivatives including esters, amides, etc.

As compounds of the present invention illustrating a useful degree of antibacterial activity, the following may be mentioned:

No. 20--6,6-dimethyl-3-(Z-methoxyphenyl)-A -8- octalone-Z-carboxylic acid No. 236,6-dimethyl-3- (4-chlorophenyl -A -8-octal0ne- Z-carboxylic acid No. 246,6-dimethyl-3-(2-methoxy-5-chloropheny1)-A 8-octalone-2-carboxylic acid These substances are variously effective in inhibiting the growth of the following organisms: Staphylococcus aurcus, Staphylococcus N0. 133, Staphylococcus pyogenes, Ncisseria gonorrheae, Diplococcus pneumoniae, Streptococcus hemolyticus, etc. The latter two substances named are superior to sulfathiazole in effectiveness against certain of these organisms. They may be administered locally in the form of solutions containing 0.2 to 10% by weight of the active ingredient.

The following substances are preferred species of the present invention as choleretic agents:

No. 166,6-dimethyl-3-phenyl-A -8-octalone-2-carboxylic acid No. 5 l6,'6-dimethyl-3 -phenyl-A -8-octalone-1,2- [N- (,8-

dimethylaminoethyl) dicar-boximide No. 176,6-dimethyl-3-(4-methoxyphenyl)-A -8- octalone-Z-carboxylic acid No. 2 l6,6-dimethyl-3- 3-methoxyphenyl -A -8- octalone-Z-carboxylic acid No. 38-6,6-dimethyl-3-phenyl-A -8-octalone-1,2-dicarboxylic acid mono-N-methylamide No. 41-6,6-dimethyl-3-phenyl-A -8-octalone-1,2-dicarboxylic acid N-tertiary butyl monoamide No. 446,6-dimethyl-3-phenyl-A -8-octalone-1,2-dicarboxylic acid N,N-diethyl monoamide No. 486,6-dimethyl-3- (2-methoxyphenyl-8-octalone- 1,2-dicarboxylic acid N,N-diethyl monoamide No. 48A6,6-dimethyl-3-(3-methoxyphenyl)-A -8-octal0ne-1,2-dicarboxylic acid N,N-diethylamide No. 376,6-dimethyl-3-phenylA -8-octalone-1,2-dicarboxylic acid monoamide The first two compounds listed, Nos. 16 and 51, are particularly preferred for their choleretic activity. Compound No. 51 is more active than sodium dchydrocholate and, in addition, is free of spasmolytic and antihistiminic side effects.

The compound of the present invention may be administered parenterally and orally for therapeutic purposes in dosages of 50-500 mg. in various of the usual pharmaceutical dosage forms including tablets, solutions, capsules, suspensions, granules, etc.

EXAMPLE A Compressed tablets The following ingredients are employed:

6,6 dimethyl 3-phenyl-A -8-octalone-1,Z-dicarboxylic acid N,N-diethyl monoamide 100 Lactose Corn starch 56.25 Talc 12.50 Magnesium stearate 1.25

The octalone is mixed with the lactose and formed into a granulation with an aqueous solution of gelatin. The dry granulation is then blended with the talc and margnesium stearate and pressed into tablets, each weighing 250 mg. and containing mg. of the active ingredient.

EXAMPLE B Solution for injection The following ingredients are employed:

G. 6,6 dimethyl 3 phenyl A -8-octalone-1,2-carboxylic acid mono-N-t-butylamide sodium salt 100 Sodium chloride 25 Distilled water, 5 l.

A solution of the ingredients is prepared, filtered to free it of foreign material, filled into ampoules, each containing 5 cc. of this solution corresponding to 100 mg. of the active ingredient, the ampoules sealed and sterilized in an autoclave.

1 1 EXAMPLE 0 0 X H l wherein R is phenyl or phenyl bearing from one to two substituents selected from alkoxy having up to four carbon atoms, halogen and hydroxyl and wherein one of X and Y is carboxyl and the other is the group in which R and R are hydrogen or lower alkyl having up to four carbon atoms.

2. A compound having the formula wherein R is phenyl or phenyl bearing from one to two substituents selected from alkoxy having up to four carbon atoms, halogen and hydroxyl and wherein one of X and Y is carboxyl and the other is the group OON in which R and R are hydrogen or lower alkyl having up to four carbon atoms.

3. The compound of claim 2 wherein R is phenyl, and R and R are ethyl.

4. The compound of claim 2 wherein R is phenyl, R is hydrogen and R is t-butyl.

5. The compound of claim 2 wherein R is 3-methoxyphenyl and R and R are ethyl.

6. The compound of claim 2 wherein R is 4-methoxyphenyl and R and R are ethyl.

References Cited UNITED STATES PATENTS 2,802,880 8/1957 Stoll et al. 260617 JAMES A. PATTEN, Primary Examiner US. Cl. X.R.

UNITILID STATES PATENT OFFICE Page 1 CERTIFICATE OF CORRECTION Patent No. 3 557, 291 Dated January 19, 197].

Inventor(s) It is certified that error appears in the above-identified patent and that said Letters Patent are hereby corrected as shown below:

Colurm 2, delete "(II-b)" from line 18 and insert it as line 26 under the bracketed formula Column 2, line 20, in both formulas, "C0O" should be co-o o umn 2, in the formula appearing at line 30, CH

COOH

should read CH 2H C IOOH Column 2, in the formula appearing between lines 45 and 50,

the structure should appear as follows:

CH CH R-CH=CH 0 Column 3, the first formula, should be part of equation (5) which appears at the bottom of Column 2 u an uncn |1n cc Sheet 2 3 557 9 2 January 19 A M-4mm ,gmmn and JALQULS JimN FuufiiSAhD Column 8, in Table 5, opposite No. 47 under the heading "N-NCH should be N-CH Column 10, line 66, "car-" at the end of the line should be dicar- Signed and sealed this 12th day of October, 1971 (SEAL) Attest:

EDWARD M.FLETCHER,JR. ROBERT GOI'TSCHALK Attesting Officer Acting Goxmnissionar of Patent: 

1. A PHARMACEUTICAL COMPOSITION IN DOSAGE UNIT FORM COMPRISING A PHARMACEUTICAL CARRIER AND FROM 50 TO 500 MG. OF A COMPOUND HAVING THE FORMULA 